http://hdl.handle.net/1893/12 This community contains the ePrints and eTheses produced by the Aquaculture staff and students. Fri, 02 Oct 2026 23:48:01 GMT 2026-10-02T23:48:01Z http://hdl.handle.net/1893/38324 Title: First Report of Snakehead Rhabdovirus-Like Novirhabdovirus Associated With Acute Mortality in Multiple Outbreaks in Farmed Giant Snakehead (Channa micropeltes) in Thailand Author(s): Yamkasem, Jidapa; Youngnoi, Natthakul; Setthawong, Piyathip; Pulpipat, Theeraporn; Surachetpong, Win; Lertwanakarn, Tuchakorn Editor(s): Ren, Linzhu Abstract: Snakehead rhabdovirus (SHRV) is a viral pathogen previously associated with mortality in snakehead fish (Channa spp.) and other closely related species. In this study, we report the detection of SHRV-like virus in giant snakehead (C. micropeltes) from multiple fish farms in Thailand, where acute disease outbreaks resulted in cumulative mortalities ranging from 7% to 90%. The affected fish showed clinical signs, including swimming near the water surface, anorexia, and skin discoloration. Histopathological examination revealed numerous eosinophilic intracytoplasmic inclusion bodies (ICIBs) in the spleen, anterior kidney, and posterior kidney. The virus was successfully isolated in E-11 cells, where it induced marked cytopathic effects (CPEs), including cell rounding, cytoplasmic vacuolization, and progressive monolayer destruction within 1–2 days post-inoculation (dpi). Transmission electron microscopy confirmed the presence of enveloped, bullet-shaped viral particles characteristic of the family Rhabdoviridae. Experimental challenge of naïve giant snakehead with the isolated virus reproduced the clinical signs observed in the field, resulted in 70% cumulative mortality within 4 days, and led to successful re-isolation of the virus from experimentally infected fish, which supports the pathogenic role of the virus in giant snakehead. Reverse-transcription polymerase chain reaction (RT-PCR) and sequence analysis demonstrated that the virus shared 79.70%–85.37% nucleotide identity with previously reported SHRV strains and placed the virus within the genus Novirhabdovirus, forming a cluster with SHRV and Carpione rhabdovirus (CAPRV). These findings provide evidence for the presence of a SHRV-like Novirhabdovirus associated with disease outbreaks in farmed giant snakehead in Thailand and highlight the need for further investigation into its epidemiology, pathogenesis, and transmission dynamics to support the development of effective disease prevention and control strategies. Thu, 06 Aug 2026 00:00:00 GMT http://hdl.handle.net/1893/38324 2026-08-06T00:00:00Z http://hdl.handle.net/1893/38313 Title: Fifty Years of Research in The Commonwealth of The Bahamas: A Quantitative Thematic Analysis of Scholarly Output Author(s): Moss, bbntbntAmina; Sherman, Krista D; Gustave, Williamson; Sullivan Sealey, Kathleen; Dunn, Charlotte; Smith, Nicola Simone; Knowles, Lindy; Gibson, Christy Abstract: This study presents the first longitudinal bibliometric mapping of peer—reviewed research on The Bahamas, analyzing 12,113 Web of Science records (1975–2025) using CiteSpace v.6.4.R2. We identified 36 co—citation clusters (modularity Q = 0.7967; silhouette = 0.9231) and 28 keyword clusters (Q = 0.7523; silhouette = 0.8695), revealing 8 thematic trajectories over 5 decades. Early research (prior to 1990) focused on carbonate geology and sea—level dynamics across the northwestern Bahama Platform and Blake—Bahama Basin (Cluster#0, silhouette = 0.939). From the 1990s–mid—2000s, attention shifted to marine fisheries, Aliger gigas, coral reefs, and ecological resilience (Cluster #1, n = 203). In the late—2000s, oceanography, microbial ecology, and stromatolitic systems were most common (Cluster #4, silhouette = 0.982). Post—2010 research encompassed public health, climate impacts, and computational methods. Burst terms (terms showing rapid, time—bounded increases in usage rather than simply the most frequent terms) including “health” (24.39), “resilience” (19.82), and “impact” (31.21) indicate rising interdisciplinarity. Persistent underrepresentation of social—ecological systems, local knowledge, and climate—governance research shows the need for transdisciplinarity, local authorship, and alignment with national development frameworks. See Supplemental Figure S1 for a graphical abstract summarizing the main findings of this study. Thu, 01 Jan 2026 00:00:00 GMT http://hdl.handle.net/1893/38313 2026-01-01T00:00:00Z http://hdl.handle.net/1893/38305 Title: Origins of Improved Hardness in Bioinspired Aragonite Precipitated Under Simulated Biologic Conditions Author(s): Chomhom, Boontharee; Farfan, Gabriela A.; Greeves, Samantha; Penkman, Kirsty; Finch, Adrian A.; Fitzer, Susan; Kröger, Roland; Brasier, Alex; Still, John; Clog, Matthieu; Allison, Nicola Abstract: Biogenic aragonite is a composite of mineral and biomolecules that exhibits superior material properties compared to its inorganic analog. This superiority underpins the success of the bioaragonite structures which support coral reefs and shell fisheries, yet the mechanisms improving the physical resilience of bioaragonite are not well understood. In this study, aragonite is synthesised under chemical conditions which simulate those of calcification sites in marine organisms. Aspartic acid, glutamic acid, and glycine in solution are incorporated into the aragonite at concentrations typically observed in marine biominerals and are observed to significantly improve aragonite Vickers hardness. Aspartic acid is incorporated more efficiently than glutamic acid or glycine, but for comparable amounts of amino acid incorporation, glycine improves aragonite hardness significantly more than either aspartic or glutamic acid. Glycine and glutamic acid incorporation reduce the aragonite grain size, thereby improving resilience to indentation via energy dissipation along grain boundaries. In contrast, aspartic acid also improves hardness on a smaller scale, possibly due to crystal structure distortions. These results suggest that amino acid incorporation influences biogenic aragonite hardness via multiple mechanisms and provide a prototype for exploring the influence of more complicated biomineral proteins. Tue, 01 Sep 2026 00:00:00 GMT http://hdl.handle.net/1893/38305 2026-09-01T00:00:00Z http://hdl.handle.net/1893/38299 Title: Cannabidiol and microbubble aeration enhance stress resilience and physiological homeostasis in European sea bass: a multi-tissue integrative approach Author(s): Ravelo, Valery; Torrecillas, Silvia; Monzón-Atienza, Luis; Acosta, Félix; Broughton, Richard; Betancor, Monica; Montero, Daniel; Fernández-Montero, Alvaro Abstract: Introduction: Animal welfare has become an increasingly important issue in the aquaculture sector, and improving it is essential for maintaining the health of farmed fish. Therefore, the aim of the present study was to evaluate the potential effect of dietary cannabidiol (CBD) supplementation (0.001%) environmental enrichment (EE) through modified aeration or their combination over 5 weeks to assess their suitability as strategies for improving welfare in aquaculture. Methods: Methods: After this period, fish were subjected to a confinement stress challenge. Welfare indicators such as survival, selected stress and immunological parameters, lipid mediators, and the expression of several stress-response and appetite-regulating related genes in different brain regions (including telencephalon, hypothalamus, and pituitary gland) were analysed in European sea bass. Results: The results showed that survival rates were significantly higher in all welfare-promoting groups; EE improved production parameters and increased dissolved oxygen levels; CBD treatments did not negatively affect growth performance, reduced plasma cortisol during both acute and adaptive stress responses, enhanced basal serum lysozyme activity, and promoted a faster post-stress stabilization; and the combined treatment supported a synergistic interaction between EE and CBD, in which lipid mediators suggested modulations of inflammatory responses under stress and helped to mitigate the effects of the stress response-related genes and a potential involvement in appetite regulation and energy balance strategies. Discussion: These results highlight the potential of microbubble curtain enrichment and CBD for improving welfare of juvenile sea bass, enhancing growth, stress resilience, and physiological regulation, with the combined treatment showing the strongest and most effective improvements. Tue, 01 Sep 2026 00:00:00 GMT http://hdl.handle.net/1893/38299 2026-09-01T00:00:00Z