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Title: Molecular Diagnostics of Ebola Patient Samples by Institut Pasteur de Dakar Mobile Laboratory in Guinea 2014-2016
Author(s): Faye, Oumar
Diagne, Cheikh Tidiane
Diallo, Amadou
Meyer, Emily
Soropogui, Barre
Fall, Gamou
Fall, Cheikh
Magassouba, N'Faly
Koivogui, Lamine
Keita, Sakoba
Loucoubar, Cheikh
Diop, Mamadou
Weidmann, Manfred
Faye, Ousmane
Sall, Amadou Alpha
Editor(s): Okware, Samuel Ikwaras
Sponsor: DFID Department for International Development
Citation: Faye O, Diagne CT, Diallo A, Meyer E, Soropogui B, Fall G, Fall C, Magassouba N, Koivogui L, Keita S, Loucoubar C, Diop M, Weidmann M, Faye O & Sall AA (2020) Molecular Diagnostics of Ebola Patient Samples by Institut Pasteur de Dakar Mobile Laboratory in Guinea 2014-2016. In: Okware SI (ed.) Re-emerging Filovirus Diseases. London: InTech, pp. 71-81.
Keywords: Ebola disease virus
West-Africa epidemic
mobile laboratory deployment
social benefits
Issue Date: 2020
Date Deposited: 30-Sep-2019
Abstract: As part of the laboratory response to the Ebola virus outbreak in Guinea, the Institut Pasteur de Dakar mobile laboratory (IPD-ML) was set up in Donka hospital from 2014 to 2016. EBOV suspected samples collected at Ebola Treatment Centers (ETC) and from community deaths were sent daily to IPD-ML. Analysis was performed using dried oligonucleotide mixes for real-time RT-PCR designed for field diagnostic. From March 2014 to May 2015, a total of 6055 patient samples suspected for EBOV collected from seven regions of Guinea were tested by real-time RT-PCR. These patients’ clinical included serum samples (n = 2537 samples) and swabs (n = 3518 samples) with positivity rates of 36.74 and 6.88% respectively. Females were significantly more affected than males with positivity rates of 22.39 and 17.22% respectively (p-value = 5.721e-7). All age groups were exposed to the virus with significant difference (p-value
Rights: © 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
DOI Link: 10.5772/intechopen.88263
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