Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/35163
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dc.contributor.authorMarrero, Manuelen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorPerez, Jose Aen_UK
dc.contributor.authorBetancor, Monicaen_UK
dc.contributor.authorGalindo, Anaen_UK
dc.contributor.authorBolanos, Anaen_UK
dc.contributor.authorAcosta, N Guadalupeen_UK
dc.contributor.authorRodriguez, Covadongaen_UK
dc.date.accessioned2023-05-30T00:05:23Z-
dc.date.available2023-05-30T00:05:23Z-
dc.date.issued2024-01en_UK
dc.identifier.other110865en_UK
dc.identifier.urihttp://hdl.handle.net/1893/35163-
dc.description.abstractThe capacity to biosynthesise long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) depends upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. The presence of a Δ5/Δ6 desaturase enabling the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) through the “Sprecher pathway” has been reported in Chelon labrosus. Research in other teleosts have demonstrated that LC-PUFA biosynthesis can be modulated by diet and ambient salinity. The present study aimed to assess the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity (35 ppt vs 20 ppt) on the fatty acid composition of muscle, enterocytes and hepatocytes of C. labrosus juveniles. Moreover, the enzymatic activity over radiolabelled [1-14C] 18:3n-3 (α-linolenic acid, ALA) and [1-14C] 20:5n-3 (eicosapentaenoic acid, EPA) to biosynthesise n-3 LC-PUFA in hepatocytes and enterocytes, and the gene regulation of the C. labrosus fatty acid desaturase-2 (fads2) and elongation of very long chain fatty acids protein 5 (elovl5) in liver and intestine was also investigated. Recovery of radiolabelled products including stearidonic acid (18:4n-3, SDA), 20:5n-3, tetracosahexaenoic acid (24:6n-3, THA) and 22:6n-3 in all treatments except FO35-fish, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA and DHA from ALA is present and active in C. labrosus. Low salinity conditions upregulated fads2 in hepatocytes and elovl5 in both cell types, regardless of dietary composition. Interestingly, FO20-fish showed the highest amount of n-3 LC-PUFA in muscle, while no differences in VO-fish reared at both salinities were found. These results demonstrate a compensatory capacity of C. labrosus to biosynthesise n-3 LC-PUFA under reduced dietary supply, and emphasise the potential of low salinity conditions to stimulate this pathway in euryhaline fish.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMarrero M, Monroig O, Perez JA, Betancor M, Galindo A, Bolanos A, Acosta NG & Rodriguez C (2024) Dietary LC-PUFA and environmental salinity modulate the fatty acid biosynthesis capacity of the euryhaline teleost thicklip grey mullet (Chelon labrosus). <i>Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology</i>, 269, Art. No.: 110865. https://doi.org/10.1016/j.cbpb.2023.110865en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Marrero M, Monroig O, Perez JA, Betancor M, Galindo A, Bolanos A, Acosta NG & Rodriguez C (2024) Dietary LC-PUFA and environmental salinity modulate the fatty acid biosynthesis capacity of the euryhaline teleost thicklip grey mullet (Chelon labrosus). Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 269, Art. No.: 110865. https://doi.org/10.1016/j.cbpb.2023.110865 © 2023, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectLC-PUFA biosynthesisen_UK
dc.subjectLow salinityen_UK
dc.subjectDieten_UK
dc.subjectChelon labrosusen_UK
dc.titleDietary LC-PUFA and environmental salinity modulate the fatty acid biosynthesis capacity of the euryhaline teleost thicklip grey mullet (Chelon labrosus)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2024-05-24en_UK
dc.rights.embargoreason[Marrero et al. 2023.pdf] Publisher requires embargo of 12 months after publication.en_UK
dc.identifier.doi10.1016/j.cbpb.2023.110865en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biologyen_UK
dc.citation.issn1879-1107en_UK
dc.citation.issn1096-4959en_UK
dc.citation.volume269en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderUniversidad de la Lagunaen_UK
dc.author.emailm.b.betancor@stir.ac.uken_UK
dc.citation.date23/05/2023en_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.identifier.wtid1907296en_UK
dc.contributor.orcid0000-0003-1626-7458en_UK
dc.date.accepted2023-05-19en_UK
dcterms.dateAccepted2023-05-19en_UK
dc.date.filedepositdate2023-05-26en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMarrero, Manuel|en_UK
local.rioxx.authorMonroig, Oscar|en_UK
local.rioxx.authorPerez, Jose A|en_UK
local.rioxx.authorBetancor, Monica|0000-0003-1626-7458en_UK
local.rioxx.authorGalindo, Ana|en_UK
local.rioxx.authorBolanos, Ana|en_UK
local.rioxx.authorAcosta, N Guadalupe|en_UK
local.rioxx.authorRodriguez, Covadonga|en_UK
local.rioxx.projectProject ID unknown|Universidad de la Laguna|http://dx.doi.org/10.13039/100015528en_UK
local.rioxx.freetoreaddate2024-05-24en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2024-05-23en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2024-05-24|en_UK
local.rioxx.filenameMarrero et al. 2023.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1879-1107en_UK
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