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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: An Efficient Method for the Plating of Haploid and Diploid Emiliania huxleyi on Solid Medium
Author(s): Skeffington, Alastair W.
Grimm, Annett
Schönefeld, Steffi
Petersen, Kerstin
Scheffel, André
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Keywords: Coccolithophore
isolation clonal strains
Issue Date: 14-Feb-2020
Date Deposited: 28-Jun-2023
Citation: Skeffington AW, Grimm A, Schönefeld S, Petersen K & Scheffel A (2020) An Efficient Method for the Plating of Haploid and Diploid Emiliania huxleyi on Solid Medium. <i>Journal of Phycology</i>, 56 (1), pp. 238-242.
Abstract: Emiliania huxleyi is a globally important coccolithophore and one of the most successful eukaryotic organisms in the modern oceans. Despite a large body of work on this organism, including the sequencing of its genome, the tools required for forward and reverse functional genetic studies are still undeveloped. Here we present an optimized method for the clonal isolation of E. huxleyi by plating on solid medium. We demonstrate the utility of this method for a variety of strains including haploid, calcifying-diploid, and noncalcifying diploid strains. We show that, in contrast to previous studies, no changes in cell ploidy status occur when the cells are plated. Our method will greatly aid attempts to elucidate the genetic basis of the remarkable physiology of E. huxleyi by forward and reverse genetic approaches.
DOI Link: 10.1111/jpy.12942
Rights: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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