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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/35790
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dc.contributor.authorRuiz Daniels, Roseen_UK
dc.contributor.authorTaylor, Richard Sen_UK
dc.contributor.authorDobie, Rossen_UK
dc.contributor.authorSalisbury, Sarahen_UK
dc.contributor.authorFurniss, James Jen_UK
dc.contributor.authorClark, Emilyen_UK
dc.contributor.authorMacqueen, Daniel Jen_UK
dc.contributor.authorRobledo, Diegoen_UK
dc.contributor.editorWinter, Svenen_UK
dc.date.accessioned2024-02-28T01:06:22Z-
dc.date.available2024-02-28T01:06:22Z-
dc.date.issued2023-09-07en_UK
dc.identifier.othere0285020en_UK
dc.identifier.urihttp://hdl.handle.net/1893/35790-
dc.description.abstractThe use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Science (PLoS)en_UK
dc.relationRuiz Daniels R, Taylor RS, Dobie R, Salisbury S, Furniss JJ, Clark E, Macqueen DJ & Robledo D (2023) A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues. Winter S (Editor) <i>PLOS ONE</i>, 18 (9), Art. No.: e0285020. https://doi.org/10.1371/journal.pone.0285020en_UK
dc.rights© 2023 Ruiz Daniels et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectMultidisciplinaryen_UK
dc.titleA versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissuesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/journal.pone.0285020en_UK
dc.identifier.pmid37676875en_UK
dc.citation.jtitlePLoS ONEen_UK
dc.citation.issn1932-6203en_UK
dc.citation.volume18en_UK
dc.citation.issue9en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.author.emailrose.ruizdaniels@stir.ac.uken_UK
dc.citation.date07/09/2023en_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.identifier.scopusid2-s2.0-85170191095en_UK
dc.identifier.wtid1975095en_UK
dc.contributor.orcid0000-0001-5643-2070en_UK
dc.date.accepted2023-09-01en_UK
dcterms.dateAccepted2023-09-01en_UK
dc.date.filedepositdate2024-02-27en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorRuiz Daniels, Rose|en_UK
local.rioxx.authorTaylor, Richard S|en_UK
local.rioxx.authorDobie, Ross|en_UK
local.rioxx.authorSalisbury, Sarah|en_UK
local.rioxx.authorFurniss, James J|0000-0001-5643-2070en_UK
local.rioxx.authorClark, Emily|en_UK
local.rioxx.authorMacqueen, Daniel J|en_UK
local.rioxx.authorRobledo, Diego|en_UK
local.rioxx.projectProject ID unknown|Biotechnology and Biological Sciences Research Council|http://dx.doi.org/10.13039/501100000268en_UK
local.rioxx.contributorWinter, Sven|en_UK
local.rioxx.freetoreaddate2024-02-27en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2024-02-27|en_UK
local.rioxx.filenamejournal.pone.0285020.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1932-6203en_UK
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