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http://hdl.handle.net/1893/35790| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues |
| Author(s): | Ruiz Daniels, Rose Taylor, Richard S Dobie, Ross Salisbury, Sarah Furniss, James J Clark, Emily Macqueen, Daniel J Robledo, Diego |
| Contact Email: | rose.ruizdaniels@stir.ac.uk |
| Keywords: | Multidisciplinary |
| Issue Date: | 7-Sep-2023 |
| Date Deposited: | 27-Feb-2024 |
| Citation: | Ruiz Daniels R, Taylor RS, Dobie R, Salisbury S, Furniss JJ, Clark E, Macqueen DJ & Robledo D (2023) A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues. Winter S (Editor) <i>PLOS ONE</i>, 18 (9), Art. No.: e0285020. https://doi.org/10.1371/journal.pone.0285020 |
| Abstract: | The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species. |
| DOI Link: | 10.1371/journal.pone.0285020 |
| Rights: | © 2023 Ruiz Daniels et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Licence URL(s): | http://creativecommons.org/licenses/by/4.0/ |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| journal.pone.0285020.pdf | Fulltext - Published Version | 666.98 kB | Adobe PDF | View/Open |
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